RT-PCR Analysis of RNA Extracted from Bouin-Fixed and Paraffin-Embedded Lymphoid Tissues
نویسندگان
چکیده
منابع مشابه
A multiplex endpoint RT-PCR assay for quality assessment of RNA extracted from formalin-fixed paraffin-embedded tissues
BACKGROUND RNA extracted from formalin-fixed paraffin-embedded (FFPE) samples is chemically modified and degraded, which compromises its use in gene expression studies. Most of the current approaches for RNA quality assessment are not suitable for FFPE derived RNA. RESULTS We have developed a single-tube multiplex endpoint RT-PCR assay specifically designed to evaluate RNA extracted from FFPE...
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ژورنال
عنوان ژورنال: The Journal of Molecular Diagnostics
سال: 2004
ISSN: 1525-1578
DOI: 10.1016/s1525-1578(10)60524-7